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blocking anti-β1 integrin antibody clone aiib2  (Millipore)


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    Millipore blocking anti-β1 integrin antibody clone aiib2
    ( a – c ) ECs increase the levels of active <t>β1</t> <t>integrin</t> in hfRPE basal plasma membrane. ( a , b ) Active β1 integrin (blue) and the TJ protein ZO-1 (red) in hfRPE were assessed by immunofluorescence assays (10 z-stacks from 2 biological replicates, t -test). Top panel, orthogonal view (xz) of a confocal z-stack. Bottom panel, confocal plane (xy) indicated by the arrowhead in the top panel. Bar, 10 μm. ( c ) Example of the relative fluorescence of individual confocal planes from 5 z-stacks is shown from the most apical (left) to the most basal (right) planes. ( d ) Inhibition of hfRPE β1 integrin with a blocking antibody impairs EC-mediated increase in TER. For clarity, only the statistical analyses at day 7 are shown ( n =6, ANOVA), all groups versus VeraVec ECs+anti-β1 integrin). ( e , f ) RhoA/ROCK and Rac1 pathways are involved in the EC-mediated increase in hfRPE TER. For clarity in e , only the statistical analyses at week 2 are shown. In e : *Mock control versus mock+ROCK inhibitor; && VeraVec ECs control versus VeraVec ECs+Rac1 inhibitor ( n =6, ANOVA). In f : ***Mock control versus mock+RhoA activator; &&& VeraVec ECs control versus VeraVec ECs+RhoA activator ( n =3, ANOVA). ( g , h ) Cell surface biotinylation assays showing EC-mediated increase in occludin cell surface localization in hfRPE ( n =4, t -test). ( i , j ) Immunofluorescence assays show a lysyl oxidase activity-dependent enhancement of occludin accumulation (green) along ZO-1-positive TJs (red) in hfRPE when exposed to choroid EC-conditioned media ( n =5, ANOVA). Bar, 20 μm. ( k ) Rac1 inhibition impairs EC-mediated occludin accumulation at hfRPE TJs [10 (mock and VeraVec ECs) or 12 (VeraVec ECs+Rac1 inhibitor) z-stacks from 2 biological replicates, ANOVA). ( l , m ) Increasing substrate stiffness enhances occludin localization at hfRPE TJs (10 z-stacks from 2 biological replicates, ANOVA). Bar, 10 μm. ( n ) Inhibiton of lysyl oxidase acitivity does not impair EC-mediated accumulation of collagen I in hfRPE basement membrane (40 images per condition from 2 biological replicates, ANOVA). Data in c – f , h and j are presented as mean±s.d.
    Blocking Anti β1 Integrin Antibody Clone Aiib2, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+anti-%CE%B21+integrin+antibody+clone+aiib2/%CE%B21+integrin+aiib2+antibody/pmc05454459-142-26-35
    Average 90 stars, based on 1 article reviews
    blocking anti-β1 integrin antibody clone aiib2 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors"

    Article Title: Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors

    Journal: Nature Communications

    doi: 10.1038/ncomms15374

    ( a – c ) ECs increase the levels of active β1 integrin in hfRPE basal plasma membrane. ( a , b ) Active β1 integrin (blue) and the TJ protein ZO-1 (red) in hfRPE were assessed by immunofluorescence assays (10 z-stacks from 2 biological replicates, t -test). Top panel, orthogonal view (xz) of a confocal z-stack. Bottom panel, confocal plane (xy) indicated by the arrowhead in the top panel. Bar, 10 μm. ( c ) Example of the relative fluorescence of individual confocal planes from 5 z-stacks is shown from the most apical (left) to the most basal (right) planes. ( d ) Inhibition of hfRPE β1 integrin with a blocking antibody impairs EC-mediated increase in TER. For clarity, only the statistical analyses at day 7 are shown ( n =6, ANOVA), all groups versus VeraVec ECs+anti-β1 integrin). ( e , f ) RhoA/ROCK and Rac1 pathways are involved in the EC-mediated increase in hfRPE TER. For clarity in e , only the statistical analyses at week 2 are shown. In e : *Mock control versus mock+ROCK inhibitor; && VeraVec ECs control versus VeraVec ECs+Rac1 inhibitor ( n =6, ANOVA). In f : ***Mock control versus mock+RhoA activator; &&& VeraVec ECs control versus VeraVec ECs+RhoA activator ( n =3, ANOVA). ( g , h ) Cell surface biotinylation assays showing EC-mediated increase in occludin cell surface localization in hfRPE ( n =4, t -test). ( i , j ) Immunofluorescence assays show a lysyl oxidase activity-dependent enhancement of occludin accumulation (green) along ZO-1-positive TJs (red) in hfRPE when exposed to choroid EC-conditioned media ( n =5, ANOVA). Bar, 20 μm. ( k ) Rac1 inhibition impairs EC-mediated occludin accumulation at hfRPE TJs [10 (mock and VeraVec ECs) or 12 (VeraVec ECs+Rac1 inhibitor) z-stacks from 2 biological replicates, ANOVA). ( l , m ) Increasing substrate stiffness enhances occludin localization at hfRPE TJs (10 z-stacks from 2 biological replicates, ANOVA). Bar, 10 μm. ( n ) Inhibiton of lysyl oxidase acitivity does not impair EC-mediated accumulation of collagen I in hfRPE basement membrane (40 images per condition from 2 biological replicates, ANOVA). Data in c – f , h and j are presented as mean±s.d.
    Figure Legend Snippet: ( a – c ) ECs increase the levels of active β1 integrin in hfRPE basal plasma membrane. ( a , b ) Active β1 integrin (blue) and the TJ protein ZO-1 (red) in hfRPE were assessed by immunofluorescence assays (10 z-stacks from 2 biological replicates, t -test). Top panel, orthogonal view (xz) of a confocal z-stack. Bottom panel, confocal plane (xy) indicated by the arrowhead in the top panel. Bar, 10 μm. ( c ) Example of the relative fluorescence of individual confocal planes from 5 z-stacks is shown from the most apical (left) to the most basal (right) planes. ( d ) Inhibition of hfRPE β1 integrin with a blocking antibody impairs EC-mediated increase in TER. For clarity, only the statistical analyses at day 7 are shown ( n =6, ANOVA), all groups versus VeraVec ECs+anti-β1 integrin). ( e , f ) RhoA/ROCK and Rac1 pathways are involved in the EC-mediated increase in hfRPE TER. For clarity in e , only the statistical analyses at week 2 are shown. In e : *Mock control versus mock+ROCK inhibitor; && VeraVec ECs control versus VeraVec ECs+Rac1 inhibitor ( n =6, ANOVA). In f : ***Mock control versus mock+RhoA activator; &&& VeraVec ECs control versus VeraVec ECs+RhoA activator ( n =3, ANOVA). ( g , h ) Cell surface biotinylation assays showing EC-mediated increase in occludin cell surface localization in hfRPE ( n =4, t -test). ( i , j ) Immunofluorescence assays show a lysyl oxidase activity-dependent enhancement of occludin accumulation (green) along ZO-1-positive TJs (red) in hfRPE when exposed to choroid EC-conditioned media ( n =5, ANOVA). Bar, 20 μm. ( k ) Rac1 inhibition impairs EC-mediated occludin accumulation at hfRPE TJs [10 (mock and VeraVec ECs) or 12 (VeraVec ECs+Rac1 inhibitor) z-stacks from 2 biological replicates, ANOVA). ( l , m ) Increasing substrate stiffness enhances occludin localization at hfRPE TJs (10 z-stacks from 2 biological replicates, ANOVA). Bar, 10 μm. ( n ) Inhibiton of lysyl oxidase acitivity does not impair EC-mediated accumulation of collagen I in hfRPE basement membrane (40 images per condition from 2 biological replicates, ANOVA). Data in c – f , h and j are presented as mean±s.d.

    Techniques Used: Clinical Proteomics, Membrane, Immunofluorescence, Fluorescence, Inhibition, Blocking Assay, Control, Activity Assay

    Related Articles

    Control:

    Article Title: Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors
    Article Snippet: Where indicated, medium in the bottom chamber of Transwell inserts was supplemented with 8 μg ml −1 control rat IgG1 (clone HRPN, cat. MABF1786) or blocking anti-β1 integrin antibody (clone AIIB2 (ref. ), cat. MABT409) (EMD Millipore).

    Blocking Assay:

    Article Title: Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors
    Article Snippet: Where indicated, medium in the bottom chamber of Transwell inserts was supplemented with 8 μg ml −1 control rat IgG1 (clone HRPN, cat. MABF1786) or blocking anti-β1 integrin antibody (clone AIIB2 (ref. ), cat. MABT409) (EMD Millipore).

    Clinical Proteomics:

    Article Title: Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors
    Article Snippet: Where indicated, medium in the bottom chamber of Transwell inserts was supplemented with 8 μg ml −1 control rat IgG1 (clone HRPN, cat. MABF1786) or blocking anti-β1 integrin antibody (clone AIIB2 (ref. ), cat. MABT409) (EMD Millipore).

    Membrane:

    Article Title: Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors
    Article Snippet: Where indicated, medium in the bottom chamber of Transwell inserts was supplemented with 8 μg ml −1 control rat IgG1 (clone HRPN, cat. MABF1786) or blocking anti-β1 integrin antibody (clone AIIB2 (ref. ), cat. MABT409) (EMD Millipore).

    Immunofluorescence:

    Article Title: Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors
    Article Snippet: Where indicated, medium in the bottom chamber of Transwell inserts was supplemented with 8 μg ml −1 control rat IgG1 (clone HRPN, cat. MABF1786) or blocking anti-β1 integrin antibody (clone AIIB2 (ref. ), cat. MABT409) (EMD Millipore).

    Fluorescence:

    Article Title: Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors
    Article Snippet: Where indicated, medium in the bottom chamber of Transwell inserts was supplemented with 8 μg ml −1 control rat IgG1 (clone HRPN, cat. MABF1786) or blocking anti-β1 integrin antibody (clone AIIB2 (ref. ), cat. MABT409) (EMD Millipore).

    Inhibition:

    Article Title: Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors
    Article Snippet: Where indicated, medium in the bottom chamber of Transwell inserts was supplemented with 8 μg ml −1 control rat IgG1 (clone HRPN, cat. MABF1786) or blocking anti-β1 integrin antibody (clone AIIB2 (ref. ), cat. MABT409) (EMD Millipore).

    Activity Assay:

    Article Title: Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors
    Article Snippet: Where indicated, medium in the bottom chamber of Transwell inserts was supplemented with 8 μg ml −1 control rat IgG1 (clone HRPN, cat. MABF1786) or blocking anti-β1 integrin antibody (clone AIIB2 (ref. ), cat. MABT409) (EMD Millipore).



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    blocking anti-β1 integrin antibody clone aiib2  (Millipore)
    90
    Millipore blocking anti-β1 integrin antibody clone aiib2
    ( a – c ) ECs increase the levels of active <t>β1</t> <t>integrin</t> in hfRPE basal plasma membrane. ( a , b ) Active β1 integrin (blue) and the TJ protein ZO-1 (red) in hfRPE were assessed by immunofluorescence assays (10 z-stacks from 2 biological replicates, t -test). Top panel, orthogonal view (xz) of a confocal z-stack. Bottom panel, confocal plane (xy) indicated by the arrowhead in the top panel. Bar, 10 μm. ( c ) Example of the relative fluorescence of individual confocal planes from 5 z-stacks is shown from the most apical (left) to the most basal (right) planes. ( d ) Inhibition of hfRPE β1 integrin with a blocking antibody impairs EC-mediated increase in TER. For clarity, only the statistical analyses at day 7 are shown ( n =6, ANOVA), all groups versus VeraVec ECs+anti-β1 integrin). ( e , f ) RhoA/ROCK and Rac1 pathways are involved in the EC-mediated increase in hfRPE TER. For clarity in e , only the statistical analyses at week 2 are shown. In e : *Mock control versus mock+ROCK inhibitor; && VeraVec ECs control versus VeraVec ECs+Rac1 inhibitor ( n =6, ANOVA). In f : ***Mock control versus mock+RhoA activator; &&& VeraVec ECs control versus VeraVec ECs+RhoA activator ( n =3, ANOVA). ( g , h ) Cell surface biotinylation assays showing EC-mediated increase in occludin cell surface localization in hfRPE ( n =4, t -test). ( i , j ) Immunofluorescence assays show a lysyl oxidase activity-dependent enhancement of occludin accumulation (green) along ZO-1-positive TJs (red) in hfRPE when exposed to choroid EC-conditioned media ( n =5, ANOVA). Bar, 20 μm. ( k ) Rac1 inhibition impairs EC-mediated occludin accumulation at hfRPE TJs [10 (mock and VeraVec ECs) or 12 (VeraVec ECs+Rac1 inhibitor) z-stacks from 2 biological replicates, ANOVA). ( l , m ) Increasing substrate stiffness enhances occludin localization at hfRPE TJs (10 z-stacks from 2 biological replicates, ANOVA). Bar, 10 μm. ( n ) Inhibiton of lysyl oxidase acitivity does not impair EC-mediated accumulation of collagen I in hfRPE basement membrane (40 images per condition from 2 biological replicates, ANOVA). Data in c – f , h and j are presented as mean±s.d.
    Blocking Anti β1 Integrin Antibody Clone Aiib2, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+anti-%CE%B21+integrin+antibody+clone+aiib2/%CE%B21+integrin+aiib2+antibody/pmc05454459-142-26-35
    Average 90 stars, based on 1 article reviews
    blocking anti-β1 integrin antibody clone aiib2 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( a – c ) ECs increase the levels of active β1 integrin in hfRPE basal plasma membrane. ( a , b ) Active β1 integrin (blue) and the TJ protein ZO-1 (red) in hfRPE were assessed by immunofluorescence assays (10 z-stacks from 2 biological replicates, t -test). Top panel, orthogonal view (xz) of a confocal z-stack. Bottom panel, confocal plane (xy) indicated by the arrowhead in the top panel. Bar, 10 μm. ( c ) Example of the relative fluorescence of individual confocal planes from 5 z-stacks is shown from the most apical (left) to the most basal (right) planes. ( d ) Inhibition of hfRPE β1 integrin with a blocking antibody impairs EC-mediated increase in TER. For clarity, only the statistical analyses at day 7 are shown ( n =6, ANOVA), all groups versus VeraVec ECs+anti-β1 integrin). ( e , f ) RhoA/ROCK and Rac1 pathways are involved in the EC-mediated increase in hfRPE TER. For clarity in e , only the statistical analyses at week 2 are shown. In e : *Mock control versus mock+ROCK inhibitor; && VeraVec ECs control versus VeraVec ECs+Rac1 inhibitor ( n =6, ANOVA). In f : ***Mock control versus mock+RhoA activator; &&& VeraVec ECs control versus VeraVec ECs+RhoA activator ( n =3, ANOVA). ( g , h ) Cell surface biotinylation assays showing EC-mediated increase in occludin cell surface localization in hfRPE ( n =4, t -test). ( i , j ) Immunofluorescence assays show a lysyl oxidase activity-dependent enhancement of occludin accumulation (green) along ZO-1-positive TJs (red) in hfRPE when exposed to choroid EC-conditioned media ( n =5, ANOVA). Bar, 20 μm. ( k ) Rac1 inhibition impairs EC-mediated occludin accumulation at hfRPE TJs [10 (mock and VeraVec ECs) or 12 (VeraVec ECs+Rac1 inhibitor) z-stacks from 2 biological replicates, ANOVA). ( l , m ) Increasing substrate stiffness enhances occludin localization at hfRPE TJs (10 z-stacks from 2 biological replicates, ANOVA). Bar, 10 μm. ( n ) Inhibiton of lysyl oxidase acitivity does not impair EC-mediated accumulation of collagen I in hfRPE basement membrane (40 images per condition from 2 biological replicates, ANOVA). Data in c – f , h and j are presented as mean±s.d.

    Journal: Nature Communications

    Article Title: Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors

    doi: 10.1038/ncomms15374

    Figure Lengend Snippet: ( a – c ) ECs increase the levels of active β1 integrin in hfRPE basal plasma membrane. ( a , b ) Active β1 integrin (blue) and the TJ protein ZO-1 (red) in hfRPE were assessed by immunofluorescence assays (10 z-stacks from 2 biological replicates, t -test). Top panel, orthogonal view (xz) of a confocal z-stack. Bottom panel, confocal plane (xy) indicated by the arrowhead in the top panel. Bar, 10 μm. ( c ) Example of the relative fluorescence of individual confocal planes from 5 z-stacks is shown from the most apical (left) to the most basal (right) planes. ( d ) Inhibition of hfRPE β1 integrin with a blocking antibody impairs EC-mediated increase in TER. For clarity, only the statistical analyses at day 7 are shown ( n =6, ANOVA), all groups versus VeraVec ECs+anti-β1 integrin). ( e , f ) RhoA/ROCK and Rac1 pathways are involved in the EC-mediated increase in hfRPE TER. For clarity in e , only the statistical analyses at week 2 are shown. In e : *Mock control versus mock+ROCK inhibitor; && VeraVec ECs control versus VeraVec ECs+Rac1 inhibitor ( n =6, ANOVA). In f : ***Mock control versus mock+RhoA activator; &&& VeraVec ECs control versus VeraVec ECs+RhoA activator ( n =3, ANOVA). ( g , h ) Cell surface biotinylation assays showing EC-mediated increase in occludin cell surface localization in hfRPE ( n =4, t -test). ( i , j ) Immunofluorescence assays show a lysyl oxidase activity-dependent enhancement of occludin accumulation (green) along ZO-1-positive TJs (red) in hfRPE when exposed to choroid EC-conditioned media ( n =5, ANOVA). Bar, 20 μm. ( k ) Rac1 inhibition impairs EC-mediated occludin accumulation at hfRPE TJs [10 (mock and VeraVec ECs) or 12 (VeraVec ECs+Rac1 inhibitor) z-stacks from 2 biological replicates, ANOVA). ( l , m ) Increasing substrate stiffness enhances occludin localization at hfRPE TJs (10 z-stacks from 2 biological replicates, ANOVA). Bar, 10 μm. ( n ) Inhibiton of lysyl oxidase acitivity does not impair EC-mediated accumulation of collagen I in hfRPE basement membrane (40 images per condition from 2 biological replicates, ANOVA). Data in c – f , h and j are presented as mean±s.d.

    Article Snippet: Where indicated, medium in the bottom chamber of Transwell inserts was supplemented with 8 μg ml −1 control rat IgG1 (clone HRPN, cat. MABF1786) or blocking anti-β1 integrin antibody (clone AIIB2 (ref. ), cat. MABT409) (EMD Millipore).

    Techniques: Clinical Proteomics, Membrane, Immunofluorescence, Fluorescence, Inhibition, Blocking Assay, Control, Activity Assay